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col ii 250 484  (Novus Biologicals)


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    Novus Biologicals col ii 250 484
    Figure 2. qRT-PCR analysis of chondrogenic genes. (A) Histogram displays the qRT-PCR analysis of collagen type <t>II</t> <t>(Col</t> II), Sox9, <t>aggrecan</t> (Acan), and Col X in a cell micromass culture in the existence of extracellular vesicles (EVs) derived from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days in vitro histological analysis. (B, a) Statistical analysis of the comparison between micromass diameters of all experimental groups and the control that demonstrated the Co-ag-EV + and MSC-ag-EV + groups show bigger size and differentiation phenotype of MSCs to chondrocytes than the control group (received chondrogenic medium contain TGFß1) and Cho-ag-EV group. (B, b) Toluidine blue was performed for (TB) of mesenchymal stem cells (MSCs) micromass sections that differentiated into chondrocytes in the presence of extracellular vesicles (EVs) harvested from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days. (B, c) Statistical analysis of TB staining based on the percent of positive staining area. TB staining does not display a statistically significant difference between the groups. (B, d) safranin O (SO) staining was performed for all micromass sections. (B, e) Statistical analysis of SO staining based on the percent of positive staining area. SO staining does not display a statistically significant difference between the groups. The results are expressed as the mean ± SEM. *Significant difference compared with the control group (*p < 0.05; **p < 0.01).
    Col Ii 250 484, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/col ii 250 484/product/Novus Biologicals
    Average 94 stars, based on 29 article reviews
    col ii 250 484 - by Bioz Stars, 2026-04
    94/100 stars

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    1) Product Images from "Co-aggregation of MSC/chondrocyte in a dynamic 3D culture elevates the therapeutic effect of secreted extracellular vesicles on osteoarthritis in a rat model."

    Article Title: Co-aggregation of MSC/chondrocyte in a dynamic 3D culture elevates the therapeutic effect of secreted extracellular vesicles on osteoarthritis in a rat model.

    Journal: Scientific reports

    doi: 10.1038/s41598-022-22592-4

    Figure 2. qRT-PCR analysis of chondrogenic genes. (A) Histogram displays the qRT-PCR analysis of collagen type II (Col II), Sox9, aggrecan (Acan), and Col X in a cell micromass culture in the existence of extracellular vesicles (EVs) derived from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days in vitro histological analysis. (B, a) Statistical analysis of the comparison between micromass diameters of all experimental groups and the control that demonstrated the Co-ag-EV + and MSC-ag-EV + groups show bigger size and differentiation phenotype of MSCs to chondrocytes than the control group (received chondrogenic medium contain TGFß1) and Cho-ag-EV group. (B, b) Toluidine blue was performed for (TB) of mesenchymal stem cells (MSCs) micromass sections that differentiated into chondrocytes in the presence of extracellular vesicles (EVs) harvested from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days. (B, c) Statistical analysis of TB staining based on the percent of positive staining area. TB staining does not display a statistically significant difference between the groups. (B, d) safranin O (SO) staining was performed for all micromass sections. (B, e) Statistical analysis of SO staining based on the percent of positive staining area. SO staining does not display a statistically significant difference between the groups. The results are expressed as the mean ± SEM. *Significant difference compared with the control group (*p < 0.05; **p < 0.01).
    Figure Legend Snippet: Figure 2. qRT-PCR analysis of chondrogenic genes. (A) Histogram displays the qRT-PCR analysis of collagen type II (Col II), Sox9, aggrecan (Acan), and Col X in a cell micromass culture in the existence of extracellular vesicles (EVs) derived from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days in vitro histological analysis. (B, a) Statistical analysis of the comparison between micromass diameters of all experimental groups and the control that demonstrated the Co-ag-EV + and MSC-ag-EV + groups show bigger size and differentiation phenotype of MSCs to chondrocytes than the control group (received chondrogenic medium contain TGFß1) and Cho-ag-EV group. (B, b) Toluidine blue was performed for (TB) of mesenchymal stem cells (MSCs) micromass sections that differentiated into chondrocytes in the presence of extracellular vesicles (EVs) harvested from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days. (B, c) Statistical analysis of TB staining based on the percent of positive staining area. TB staining does not display a statistically significant difference between the groups. (B, d) safranin O (SO) staining was performed for all micromass sections. (B, e) Statistical analysis of SO staining based on the percent of positive staining area. SO staining does not display a statistically significant difference between the groups. The results are expressed as the mean ± SEM. *Significant difference compared with the control group (*p < 0.05; **p < 0.01).

    Techniques Used: Quantitative RT-PCR, Derivative Assay, In Vitro, Comparison, Control, Staining



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    col ii 250 484  (Novus Biologicals)
    94
    Novus Biologicals col ii 250 484
    Figure 2. qRT-PCR analysis of chondrogenic genes. (A) Histogram displays the qRT-PCR analysis of collagen type <t>II</t> <t>(Col</t> II), Sox9, <t>aggrecan</t> (Acan), and Col X in a cell micromass culture in the existence of extracellular vesicles (EVs) derived from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days in vitro histological analysis. (B, a) Statistical analysis of the comparison between micromass diameters of all experimental groups and the control that demonstrated the Co-ag-EV + and MSC-ag-EV + groups show bigger size and differentiation phenotype of MSCs to chondrocytes than the control group (received chondrogenic medium contain TGFß1) and Cho-ag-EV group. (B, b) Toluidine blue was performed for (TB) of mesenchymal stem cells (MSCs) micromass sections that differentiated into chondrocytes in the presence of extracellular vesicles (EVs) harvested from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days. (B, c) Statistical analysis of TB staining based on the percent of positive staining area. TB staining does not display a statistically significant difference between the groups. (B, d) safranin O (SO) staining was performed for all micromass sections. (B, e) Statistical analysis of SO staining based on the percent of positive staining area. SO staining does not display a statistically significant difference between the groups. The results are expressed as the mean ± SEM. *Significant difference compared with the control group (*p < 0.05; **p < 0.01).
    Col Ii 250 484, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/col ii 250 484/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    col ii 250 484 - by Bioz Stars, 2026-04
    94/100 stars
    		  Buy from Supplier

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    Figure 2. qRT-PCR analysis of chondrogenic genes. (A) Histogram displays the qRT-PCR analysis of collagen type II (Col II), Sox9, aggrecan (Acan), and Col X in a cell micromass culture in the existence of extracellular vesicles (EVs) derived from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days in vitro histological analysis. (B, a) Statistical analysis of the comparison between micromass diameters of all experimental groups and the control that demonstrated the Co-ag-EV + and MSC-ag-EV + groups show bigger size and differentiation phenotype of MSCs to chondrocytes than the control group (received chondrogenic medium contain TGFß1) and Cho-ag-EV group. (B, b) Toluidine blue was performed for (TB) of mesenchymal stem cells (MSCs) micromass sections that differentiated into chondrocytes in the presence of extracellular vesicles (EVs) harvested from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days. (B, c) Statistical analysis of TB staining based on the percent of positive staining area. TB staining does not display a statistically significant difference between the groups. (B, d) safranin O (SO) staining was performed for all micromass sections. (B, e) Statistical analysis of SO staining based on the percent of positive staining area. SO staining does not display a statistically significant difference between the groups. The results are expressed as the mean ± SEM. *Significant difference compared with the control group (*p < 0.05; **p < 0.01).

    Journal: Scientific reports

    Article Title: Co-aggregation of MSC/chondrocyte in a dynamic 3D culture elevates the therapeutic effect of secreted extracellular vesicles on osteoarthritis in a rat model.

    doi: 10.1038/s41598-022-22592-4

    Figure Lengend Snippet: Figure 2. qRT-PCR analysis of chondrogenic genes. (A) Histogram displays the qRT-PCR analysis of collagen type II (Col II), Sox9, aggrecan (Acan), and Col X in a cell micromass culture in the existence of extracellular vesicles (EVs) derived from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days in vitro histological analysis. (B, a) Statistical analysis of the comparison between micromass diameters of all experimental groups and the control that demonstrated the Co-ag-EV + and MSC-ag-EV + groups show bigger size and differentiation phenotype of MSCs to chondrocytes than the control group (received chondrogenic medium contain TGFß1) and Cho-ag-EV group. (B, b) Toluidine blue was performed for (TB) of mesenchymal stem cells (MSCs) micromass sections that differentiated into chondrocytes in the presence of extracellular vesicles (EVs) harvested from chondrocyte aggregates (Cho-ag-EV) and MSC aggregates (MSC-ag-EV) and Co-aggregates (Co-ag-EV) in chondrogenic medium with ( +) and without (−) TGFß1 after 21 days. (B, c) Statistical analysis of TB staining based on the percent of positive staining area. TB staining does not display a statistically significant difference between the groups. (B, d) safranin O (SO) staining was performed for all micromass sections. (B, e) Statistical analysis of SO staining based on the percent of positive staining area. SO staining does not display a statistically significant difference between the groups. The results are expressed as the mean ± SEM. *Significant difference compared with the control group (*p < 0.05; **p < 0.01).

    Article Snippet: To assess the chondrogenic proteins expression level in the extracted EVs, we also executed a wet western blot analysis with the primary antibodies of COL II (COL2A1, 250,484, Abbiotec, USA), aggrecan (ACAN, NB600-504, Novus Biologicals, USA), and COL X (COL10A, ab58632, Abcam, UK).

    Techniques: Quantitative RT-PCR, Derivative Assay, In Vitro, Comparison, Control, Staining